XYL3A_ASPJA
  Name and origin · Biochemical properties · Enzyme annotation · Literature · Protein features · Sequences · Cross-refs · Entry history
Name and origin
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Gene Name
Name: xyl3A
Other given name: xylA
Protein names
Common name: Beta-xylosidase
EC systematic name: 4-beta-D-xylan xylohydrolase
Other name: xylobiase; beta-D-xylopyranosidase; xylan 1,4-beta-xylohydrolase; exo-1,4-xylosidase; exo-1,4-beta-D-xylosidase; 1,4-beta-D-xylan xylohydrolase
Organism
Species: Aspergillus japonicus
Strain: MU-2
Taxonomic identifier: 34381
Taxonomic lineage: Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Trichocomaceae › mitosporic Trichocomaceae › Aspergillus
Enzyme activity Catalyzes the hydrolysis of the beta-1,4-xylosidic bond holding xylose monomers together in xylobiose.
Biochemical properties
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General properties
Expression host
native
Substrate
pNP-beta-D-xylopyranoside
Assay
pNP-releasing assay
Temperature Optimum(°C)
70
Temperature Stability(°C)
60
pH Optimum
4.0
pH Stability
2.0-7.0
Kinetic properties
Host Substrate Activity assay conditions Specific activity Relative activity(%) Assay Reference
Activity assay
native pNP-beta-D-xylopyranoside 0.1M acetate buffer, pH 4.5, 60°C, 410nm. 112µmol/min/mg 100 pNP-releasing assay.
native pNP-beta-D-glucopyranoside 0.1M acetate buffer, pH 4.5, 60°C, 410nm. active 32 pNP-releasing assay.
native pNP-alpha-L-arabinofuranoside 0.1M acetate buffer, pH 4.5, 60°C, 410nm. active 18 pNP-releasing assay.
native xylobiose 0.1M acetate buffer, pH 4.5, 60°C, (50mM acetate buffer, pH 4.5, 50°C for product analysis). active 4 Somogyi-Nelson assay and product analysis.
native xylotriose 0.1M acetate buffer, pH 4.5, 60°C (50mM acetate buffer, pH 4.5, 50°C for product analysis). active 6 Somogyi-Nelson assay and product analysis.
Pichia pastoris GS115 pNP-beta-D-xylopyranoside 0.1M acetate buffer, pH 4.5, 60°C, 410nm. 19µmol/min/mg Somogyi-Nelson assay.
 
Host Substrate Activity assay conditions Product formed Product analysis Reference
Product analysis
native xylobiose 0.1M acetate buffer, pH 4.5, 60°C, (50mM acetate buffer, pH 4.5, 50°C for product analysis). xylose thin layer chromatography
native xylotriose 0.1M acetate buffer, pH 4.5, 60°C (50mM acetate buffer, pH 4.5, 50°C for product analysis). xylose thin layer chromatography
 
Host Substrate Kinetic assay conditions Km Kcat(s-1) Vmax Reference
Kinetic assay
 
Other features
- Multifunctional beta-xylosidase with some beta-glucosidase and alpha-L-arabinofuranosidase activities.
- Weak activity towards oat-spelt xylan, birchwood xylan or cellobiose.
- No activity towards carboxymethyl cellulose, Avicel, soluble starch, sucrose, maltose, lactose
Enzyme annotation
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Enzyme
   Enzyme commission
3.2.1.37; 4-beta-D-xylan xylohydrolase
Gene Ontology
GO ID Term Evidence Reference
   Biological process
GO:0045493 xylan catabolic process IC
   Molecular function
GO:0009044 xylan 1,4-beta-xylosidase activity IDA
   Cellular component
GO:0005576 extracellular region IDA
Literature
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[1] Purification and properties of an extracellular beta-xylosidase from Aspergillus japonicus and sequence analysis of the encoding gene.  
Author Wakiyama M, Yoshihara K, Hayashi S and Ohta K
Journal J. Biosci. Bioeng. 2008 Oct;106(4):398-404.
Address Department of Applied Chemistry, Faculty of Engineering, University of Miyazaki, 1-1 Gakuen Kibanadai Nishi, Miyazaki 889-2192, Japan.
Abstract An extracellular protein exhibiting beta-xylosidase activity was purified from the culture filtrate of a filamentous fungus, Aspergillus japonicus strain MU-2, grown on oat spelt xylan. The purified enzyme was a monomeric glycoprotein with an apparent M(r) of 113.2 kDa as estimated by SDS-PAGE. beta-Xylosidase activity was optimal at pH 4.0 and 70 degrees C. The enzyme also showed beta-glucosidase and alpha-l-arabinofuranosidase activities. The genomic DNA and cDNA encoding this protein were cloned and sequenced. Southern blot analysis indicated that the beta-xylosidase gene (xylA) was present as a single copy in the genome. An open reading frame, consisting of 2412 bp, was not interrupted by introns, and it encoded a presumed signal peptide of 17 amino acids and a mature protein of 787 amino acids. The deduced amino acid sequence of the xylA gene product showed a high degree of identity (69%) to the primary structure of the Aspergillus niger beta-xylosidase XlnD that belongs to the glycoside hydrolase family 3. Moreover, the xylA gene was functionally expressed in the yeast Pichia pastoris.
Protein features
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Signal Peptide (Predicted) SP(17aa)
N-Terminal (Experimental)
Structure PMID Reference
Sequence PMID Reference
CBM
Glycosylation N-glycosylation (approximately 28% of total protein mass); 32.4% neutral carbohydrate content (determined using phenol-sulfuric acid method).
Other Domains
Domain Order
CAZy family GH3
Sequences
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Length 804aa
Molecular Weight in kDa (experimental) 113 (native enzyme; SDS-PAGE), 82 (native enzyme deglycosylated with N-glycosidase F; SDS-PAGE), 188 (recombinant enzyme; SDS-PAGE)
Molecular Weight in kDa (predicted) 84.64
Protein Sequence B6EY09
MAVAALALLA LLPQALGQHN SSYVDYNVEA NPDLFPQCLD TISLSFPDCQ SGPLSKNLVC DSTASPYDRA AALVSLFTLE ELIANTGNTS PGVPRLGLPP YQVWSEALHG LARANFTDNG AYSWATSFPS PILSAAAFNR TLINQIASII STQGRAFNNA GRFGLDVYSP NINTFRHPVW GRGQETPGED AYTLTAAYAY EYITGIQGGV NPEHLKLAAT AKHFAGYDIE NWDNHSRLGN DVNITQQDLA EYYTPQFLVA ARDAHVHSFM CSYNAVNGVP SCSNTFFLQT LLRDTFSFVD HGYVSGDCGA VYGVFNPHGY AANEPSAAAD AILAGTDIDC GTSYQYHFNE SITTGAVARD DIERGFIRLY ANLVELGYFD GNSSSSNPYR SLGWPDVQKT DAWNISYEAA VEGIVLLKND GTLPLASPSE GKNKSIALIG PWANATTQLQ GNYYGDAPYL ISPVDAFTAA GYTVHYAPGT EISTNSTANF SAALSAARAA DTIVFLGGID NTIEAEAQDR SSIAWPGNQL ELISQLAAQK SDDQPLVVYQ MGGGQVDSSA LKSNAKVNAL LWGGYPGQSG GLALRDILTG ARAPAGRLTT TQYPAAYAES FSALDMNLRP NETTQNPGQT YMWYTGEPVY AFGHGLFYTT FNASSAQAAK TKYTFNITDL TSAAHPDTTT VGQRTLFNFT ASITNSGQRD SDYTALVYAN TSTAGPSPYP NKWLVGFDRL AAVAKEGGTA ELNVPVAVDR LARVDEAGNT VLFPGRYEVA LNNEREVVVE VELVGEQVVL LKWPEEVQGV AGDE
DNA Sequence AB379633
GAATTCCCGT AAGATGTTCT TGACCTCCAG ACTGTGCCGA GGTCATTCGC TCGCGATGCT TATGGAAAGT GAAGTGCATA GGAGGGTTGG TCAGAGCATG ATCCGAGTGT CCGTCCGAGA GTGCTCAGAA AACTTGCGCA TTGGATTGTC CTAAATGAGA TACATGGTTA GCAAATAGTT CAGACATTAG CCTGTCCAAG GTGCGGGCCT CACCGACTCA ATATTTTACC ATCTCGTGTC AGTAGGCTGC TGCTGGAGGT ACAGGAACTG CCGAGAGGAC CGGTAGATGT AGTGTTGTGG AAGAATCGCA CCACATCGGA TGATCTCCGA CAAGGTCATT TAACCGACTG TGACGCATTT CTCTGCAGAC TCCTGCAAGG TTCTTCTCCT CCTCTCCTCC TCAACTCCTC CTCAACTCCT CCTCAACTCC TCTCCGGCTA AAGGTAAATC AAAGCAACAT GAAGCTAGGC AAGCTACGGA AGGATTCAGG CTAAAGGAGG GAGGGAAGGT GATCGACACG CCTCTTGCAA CGCATCGCCG GTTCTCCGCA GCCACCCCGC ATCTATACTC GACTCCATTC AACTCGCCTC GACTCAACTA TAAATTCCTT CACGTTTGCC GGCACAATTG AACATTGAGT ATGACGTTGT CGCCCAATTT CCCCAATTCG TCTCATCCTC CTCCCAATCA CAACCACAAC CATCACCATG GCTGTGGCGG CTCTTGCTCT GCTGGCTCTA CTGCCTCAAG CTCTGGGGCA ACATAACAGC AGCTACGTGG ATTACAACGT CGAAGCCAAT CCGGACTTGT TTCCACAATG TCTAGACACA ATCTCCCTGT CCTTCCCCGA CTGCCAGAGC GGTCCTCTGA GCAAGAACCT CGTCTGCGAC TCGACTGCCT CGCCCTATGA CCGCGCCGCG GCTCTGGTCT CCCTCTTCAC CCTCGAGGAA CTTATCGCCA ACACTGGTAA CACCAGCCCG GGTGTCCCTC GTCTGGGTCT GCCTCCATAC CAGGTCTGGA GTGAGGCCCT GCATGGCCTG GCTCGCGCCA ACTTCACCGA CAACGGGGCT TACAGCTGGG CGACGTCCTT CCCCTCACCC ATTCTCTCCG CAGCGGCCTT CAATCGCACC CTGATCAACC AGATCGCCTC CATTATTTCG ACTCAGGGCC GTGCCTTCAA CAACGCCGGC CGCTTTGGCC TCGACGTCTA CTCGCCAAAC ATCAATACCT TCCGCCATCC AGTCTGGGGT CGTGGACAGG AAACTCCTGG CGAGGATGCG TACACTCTTA CGGCCGCCTA CGCCTACGAA TACATCACGG GTATCCAGGG TGGTGTGAAC CCAGAGCATC TGAAGCTCGC CGCGACAGCC AAGCACTTTG CCGGCTACGA CATCGAGAAC TGGGACAACC ACTCCCGGCT GGGGAACGAT GTCAACATTA CGCAGCAAGA CCTGGCCGAG TACTACACGC CGCAGTTCCT CGTCGCCGCG CGCGACGCCC ACGTCCACAG CTTCATGTGC TCCTACAACG CCGTTAACGG AGTGCCCAGC TGCTCCAACA CCTTCTTTCT GCAGACCCTC CTGCGCGACA CCTTCTCCTT CGTCGACCAC GGCTACGTCT CCGGCGACTG CGGCGCCGTC TACGGCGTCT TCAACCCCCA CGGCTACGCG GCCAACGAGC CCAGCGCCGC CGCCGATGCC ATCCTCGCCG GCACTGACAT TGACTGCGGC ACCTCCTATC AATATCACTT CAACGAGTCC ATCACCACCG GGGCTGTCGC CCGCGACGAC ATCGAGCGTG GTTTCATCCG GCTGTACGCC AACCTCGTCG AGCTGGGCTA CTTCGACGGC AACAGCAGCA GCAGCAACCC GTACCGCAGC CTGGGCTGGC CCGACGTCCA GAAGACAGAC GCATGGAACA TTTCCTACGA GGCGGCAGTC GAAGGCATCG TCCTCCTGAA GAACGACGGC ACCCTCCCTC TTGCCTCCCC CTCCGAGGGC AAGAACAAAT CCATCGCCCT CATCGGCCCC TGGGCCAACG CCACCACCCA GCTCCAGGGT AACTACTACG GCGACGCGCC ATACCTCATC AGCCCGGTCG ACGCCTTCAC GGCCGCCGGG TACACAGTAC ACTACGCCCC CGGCACGGAG ATCTCGACGA ACTCGACGGC GAACTTCAGC GCCGCGCTCT CCGCGGCGCG CGCCGCCGAC ACCATCGTAT TCTTGGGGGG CATCGATAAC ACCATCGAAG CCGAAGCCCA AGACCGCAGC TCGATCGCCT GGCCCGGCAA CCAACTCGAG CTGATCTCGC AACTCGCGGC GCAGAAATCC GACGACCAGC CCCTGGTGGT GTACCAGATG GGCGGCGGCC AGGTCGACTC CTCCGCCCTC AAATCCAACG CGAAGGTCAA CGCCCTCCTC TGGGGCGGCT ACCCGGGCCA ATCCGGCGGC CTCGCCCTGC GCGACATCCT CACGGGCGCG CGCGCCCCCG CCGGCCGCCT CACCACGACC CAGTACCCGG CCGCCTACGC CGAGAGCTTC TCGGCCCTCG ACATGAACCT GCGGCCGAAT GAGACTACAC AGAACCCGGG CCAGACCTAC ATGTGGTACA CCGGCGAGCC CGTCTACGCC TTCGGCCACG GCCTGTTCTA CACCACCTTC AACGCTTCCT CAGCCCAAGC AGCGAAGACG AAGTATACCT TCAACATCAC CGACCTCACC TCCGCCGCAC ACCCAGACAC CACGACCGTC GGCCAACGCA CCCTCTTCAA CTTCACAGCC TCCATCACGA ACTCCGGACA GAGGGATTCC GATTACACCG CCCTGGTGTA CGCCAACACC TCGACTGCGG GCCCCTCCCC GTACCCGAAT AAATGGCTCG TCGGGTTCGA CCGGCTCGCC GCCGTGGCGA AGGAGGGCGG CACGGCCGAG TTGAATGTGC CGGTGGCGGT GGATCGGTTG GCGAGGGTGG ATGAAGCGGG TAACACCGTG CTGTTTCCGG GGCGGTATGA GGTGGCCCTG AATAATGAGC GCGAGGTCGT GGTCGAGGTG GAGTTGGTGG GTGAGCAGGT TGTGCTGTTG AAGTGGCCGG AGGAGGTGCA GGGGGTGGCG GGGGATGAGT AGGTTGTTTC CCGGGGGATC CCCAAAGGGA TTAATTAGGG TTGTAGGATC GGATATATAG CACTAGACCG TTACCACCAG GTGATATGCA GAATGAATGC AAAAGTTACA CATTCGGGAT TTTCAATGCA GTGACGTTGG GTTCCTTTCC TTTCTCTGAG AGCAAGCAAG CAAGAATAAC ACGCTATGTA CAGTACACAC TCCTCCCCAG GCACTAGTCA AAGATGATTG ATAATCATGA ACAGAAAAAG TTAAACGCCC TCCGTGCAAA TTTTGGTCTA TATTCGACAA CATACTCCGG GAGGGGGGGG GGGGGGATCA AAAGCCCATT AGCTCACCCG GCCGTTACGG TCCTAGTAAT GACAACACCT CCCTAAATAC TAGACAGACC TACCGCTTCT CCTTCTCCTT CTCCTTCCCC CCACTCCGCA CCTCCCGCTC CAACACATCC AACGCCTCCG CATACCGCGC GCGCGCCGCC TTCATCCCCT CCAGACTAAC CGCCACCCTA TGCACCTTCT GCGGGCGGTC CCCACCGTCC GCCTCGACGC GCACGATCTG CCCGTCCGCG GCGCCGCCGA TCTGCTGCGC GAACCACTTG CCCTCGCGGT GGGCGACGAG GGTCCAGGCC GCGCCGGCCT GGCCGGCGCG CGCCGTGCGG CCCACGCGGT GCACGTAGGT CGTCACGCTG GTCGGCACGT CGTAGTTGAC CACGTGCGTC AGCCGGGGCA GGTCGAGGCC GCGCGAGGCG CGGTCCGTCG CGATGATGAT CGCGAGCTTG CCGCGGCGGT AGGCGGTCAG GGTCTTGCGC GAGGCTGAGG ATTTGTTGGA CTTGATGATC GTGCCCGTGC GCGACGCGAG GGTCGGGTCT AGTAGCGCGA GGAGGCGCGC GAGACGCGAG GCGGATTCGG AGGATTTTGT GAAGATGAGG ACGGTCGGCG TGGTTTTGTT GGTGTTGGAG GAGGGGGCGG GGCGGGGGCG GGTGGGTTTG GTTCGGGAGG TGGTGGAGGC GCTGGAGGAG GAGCTGTCGC TGTCGCTGTC GCTGTCGGAG GAGGACTCGC TGGAGGAAGA ATCAGACTCG CTGGAGGAGG TGTCGTCTGA TTCGGAGTCC GAGCCTGAGC TGGATGTGTC CGAGTCTGAG TCCGAATCCG AATCCGAATC GTCCGAGCTG GACTCGTCCG AGGAGGTTTC GTCGGAGGAT GTGTCGTCCG AAGAGGTATC GTCCGAAGTG TCCGAATCGG AGGCCTTGGC CGCTTGGTTG GTGCCGTTGA TTCGGATGTG GGAAAGGAGA AGGCGCAGCA GGTAGAGTGG CTTCAGGGAC CCATCGCCCA CCGAGATCGA GTGCTCCTTC AAGGTCGGCG GCAGGGTGAA CTGCTCGTCG GCGCCGGCGA TCACGCCACT CTCGTCGTCG
Cross-references
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GenBank AB379633
UniProt B6EY09
Protein BAG82824
RefSeq Protein
Broad
PDB
Entry history
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Entry Name XYL3A_ASPJA
Previous Entry Names
Last Modification Date 2016-07-25