XYN11B_PENPU
  Name and origin · Biochemical properties · Enzyme annotation · Literature · Protein features · Sequences · Cross-refs · Entry history
Name and origin
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Gene Name
Name: xyn11B
Other given name: xynB
Protein names
Common name: Xylanase
EC systematic name: 4-beta-D-xylan xylanohydrolase
Other name: endo-(1-4)beta-xylan 4-xylanohydrolase; endo-1,4-xylanase; beta-1,4-xylanase; endo-beta-1,4-xylanase; endo-1,4-beta-D-xylanase; 1,4-beta-xylan xylanohydrolase; beta-D-xylanase; 4-beta-D-xylan xylanohydrolase
Organism
Species: Penicillium purpurogenum
Strain: ATCC No. MYA-38
Taxonomic identifier: 28575
Taxonomic lineage: Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Trichocomaceae › mitosporic Trichocomaceae › Penicillium
Enzyme activity Catalyzes the endo-hydrolysis of beta-1,4-xylosidic linkages in xylans
Biochemical properties
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General properties
Expression host
native
Substrate
birchwood xylan
Assay
Dinitrosalicylic acid method
Temperature Optimum(°C)
50
Temperature Stability(°C)
40
pH Optimum
3.5
pH Stability
4.5-5.5
Kinetic properties
Host Substrate Activity assay conditions Specific activity Relative activity(%) Assay Reference
Activity assay
native birchwood xylan pH 5.3, 50°C. 199µmol/min/mg 100 Dinitrosalicylic acid method.
native oat-spelt xylan pH 5.3, 50°C. 358µmol/min/mg 180 Dinitrosalicylic acid method.
native wheat-straw arabinoxylan pH 5.3, 50°C. 338µmol/min/mg 170 Dinitrosalicylic acid method.
native wheat-straw arabino-glucuronoxylan pH 5.3, 50°C. 376µmol/min/mg 189 Dinitrosalicylic acid method.
native oat-spelt xylan not specified. active Zymogram.
native birchwood xylan 50mM citrate buffer, pH 5.3, 50°C. active Zymogram.
 
Host Substrate Activity assay conditions Product formed Product analysis Reference
Product analysis
 
Host Substrate Kinetic assay conditions Km Kcat(s-1) Vmax Reference
Kinetic assay
 
Other features
- 60% activity inhibition by Hg2+.
- Complete inhibition by N-bromosuccinimide or SDS.
Enzyme annotation
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Enzyme
   Enzyme commission
3.2.1.8; 4-beta-D-xylan xylanohydrolase
Gene Ontology
GO ID Term Evidence Reference
   Biological process
GO:0045493 xylan catabolic process IDA
   Molecular function
GO:0031176 endo-1,4-beta-xylanase activity IDA
   Cellular component
GO:0005576 extracellular region IDA
Literature
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[1] Penicillium purpurogenum produces several xylanases: purification and properties of two of the enzymes.  
Author Belancic A, Scarpa J, Peirano A, Díaz R, Steiner J and Eyzaguirre J
Journal J. Biotechnol. 1995 Jul 15;41(1):71-9.
Address Laboratorio de Bioquímica, Pontificia Universidad Católica de Chile, Santiago.
Abstract The fungus Penicillium purpurogenum produces several extracellular xylanases. The two major forms (xylanases A and B) have been purified and characterized. After ammonium sulfate precipitation and chromatography in Bio-Gel P 100, xylanase A was further purified by means of DEAE-cellulose, hydroxylapatite and CM-Sephadex, and xylanase B by DEAE-cellulose and CM-Sephadex. Both xylanases showed apparent homogeneity in SDS-polyacrylamide gel electrophoresis. Xylanase A (33 kDa) has an isoelectric point of 8.6, while xylanase B (23 kDa) is isoelectric at pH 5.9. Antisera against both enzymes do not cross-react. The amino terminal sequences of xylanases A and B show no homology. The results obtained suggest that the enzymes are produced by separate genes and they may perform different functions in xylan degradation.
[2] Differences in expression of two endoxylanase genes (xynA and xynB) from Penicillium purpurogenum.  
Author Chávez R, Schachter K, Navarro C, Peirano A, Aguirre C, Bull P and Eyzaguirre J
Journal Gene 2002 Jun 26;293(1-2):161-8.
Address Laboratorio de Bioquímica, Departamento de Genética Molecular y Microbiología, Pontificia Universidad Católica de Chile, Casilla 114-D, Santiago, Chile.
Abstract A number of xylanolytic microorganisms secrete to the medium several molecular forms of endoxylanases. The physiological function of these isoforms is not clear; one possibility is that they are produced under different growth conditions. To study this problem, we have used two endoxylanases (XynA and XynB) produced by the fungus Penicillium purpurogenum. These enzymes have been previously purified and characterized; they belong to family 10 and 11 of the glycosyl hydrolases, respectively. The promoters of the xynA and xynB genes have been sequenced; both present consensus sequences for the binding of the carbon catabolite repressor CreA, but otherwise show substantial differences. The xynB promoter has eight boxes in tandem for the binding of the XlnR activator and lacks the consensus sequence for the PacC pH regulator. On the other hand, the xynA promoter contains one XlnR box and three PacC consensus sequences. To investigate if these differences are reflected in gene expression, Northern blot assays were carried out. The xynA gene is transiently expressed when oat spelt xylan is used as carbon source, but negligible expression was observed with birchwood xylan, xylose or xylitol. In contrast, xynB is broadly induced by all these carbon sources; this may be related to the presence of several XlnR boxes. Similar results were obtained by zymogram analysis of the expressed proteins. The different induction capabilities of birchwood and oat spelt xylan may be due to differences in their composition and structure. Expression assays carried out at different pH reflects that, despite the lack of PacC binding sites in the xynB promoter, this gene is tightly regulated by pH. The findings described here illustrate new and important differences between endoxylanases from families 10 and 11 in P. purpurogenum. They may help explain the production of multiple endoxylanase forms by this organism.
Protein features
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Signal Peptide (Predicted) SP(16aa)
N-Terminal (Experimental) 25aa
Structure PMID Reference
Sequence PMID Reference
CBM
Glycosylation
Other Domains
Domain Order
CAZy family GH11
Sequences
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Length 208aa
Molecular Weight in kDa (experimental) 23 (native enzyme; SDS-PAGE)
Molecular Weight in kDa (predicted) 20.31
Protein Sequence Q96W72
MKVTAAFAGL LATTLAAPAT ELVTRSINYV QNYNGNLGAF SYNEGAGTFS MYWQQGVSND FVVGLGRSTG SSNPITYSAS YSASGGSYLA VYGWVNSPQA EYHVVEAYGN YNPCSSGSAT NLGTVSSDGG TYQVCTDTRV NQPSITGTST FTQFFSVRQG SRTSGTVTIA NHFNFWAKHG FGNSNFNYQV VAVEAWSGTG TASVTVSA
DNA Sequence AF359553
CCTATTAATT AGGTAGGAGA AATAAAACTA ATTAATTATA TAAAAATAGT TAAAGACTAT GTCTTAAGGA ACGCAAGGGT TCCTCTTTAT AGTTTAATTT ATATACAATC TTATCGCTAA AAACAGGAAG GAGATATACC CTTAGGAGCG GGGGAAAAAC ATAAGGCTTA TGTAACCTCT TCCTAAGGAT CTTCCCTGTA GAGTTTCGTA CACGTGTTAG CAGCGAAGCT CTAGCGAATC CCCACCGAAA GCCTCCCGGC TTTTCAGGTC CGGGTAGCCC GAGGCACCAA CACTTTGGGA ATAACCTCAG GCCAGGGTAT ATCATAACAC TTTTAAACTA GAGACTATCA AAAATGCTTT CGCTATAGCT AGTCTAGTCC TATACGAGCT AGATCAAGTT ATTTTAAAGC TTAATGTTAA CTTCGAACAC CGATTAGTAT AATTATAATC ATTTTAAGTG GAAATACGCA TTACGAAGAT TCGACGAATA TTCATAGACT TACGAATATA TTATTCGTAA TCTTAATTCG TAATTACGAA TATTCGTAGT AGCCCTATAA GTTTTTTTTC TAGGTAGATT AAGTCAGTGT CATATAGTTT GTCCTATTTA TTATCTATAG ATATCTATGG ATTTTATAGA TACTGCCGTA GCCTAGGGTG GAGGGTGGAC TGACCTTTTA CGTTCTCGGT AGTGTTAAAA CTAAGGTCAA AAACTCGAGT TAAATAGCAT TAGGTTACGG AGACAAGAGA GTCACGATCA GCTATAGTAC ACAGGGTCTT CTCTAACTGC TAGCAGTGAT CATGAGTTCT GAGCCGGCAA AATTTCTGTG GGGAACCCGT GAAGAATCCC CACTTCCCCG CCTCCGCACT TTCTACCCAA CTCTTAGCCA ATTACAGTTC TTTTAGCCAA CTGCTATCCT TTTAGCCAAC TGCCATCATT TTAGCCATCT GCCATCCTTT TAGCCAACTG CCATCCTTTT AGCCAACTGC CATCCTTTTA GCCAACTACA GTCCTTTTAG CCAACTACAG TCCTTTTAGC CAACTACAGT GCTGAGACCG GTAGCTACAC CGTAGACCCC GACCTATAGA ACTGTCCCTA GAAACAGGCT TAGAACAAAC TCTAATCGTT CAACTGCTAA AGGTCACGCA GCAAAACGTA TATATCCGAA CCGCTTCGCC TTGCGGTGAA AAAGGATAAA TAGTCATTTT CGCAGAATAT AAATAGAGGT AGAACGGACT TGCGGCTTCT CTTCCAACTT CACACATCCA TTCACTATCA TTCAATTCTC ATCAATCATC ATGAAGGTCA CTGCAGCTTT CGCAGGCCTC TTGGCCACGA CACTCGCCGC CCCTGCCACC GAGCTAGTGA CGCGAAGTAT CAACTACGTC CAGAACTACA ACGGCAACCT TGGTGCCTTT AGCTACAACG AGGGTGCCGG AACATTCTCT ATGTACTGGC AGCAAGGAGT CAGCAACGAT TTCGTTGTTG GTTTAGGCAG GAGCACTGGT TCCTCTAAGT AGATGGCCAT ATCCTTTAAC ATCAGTCTAG GATCTAACGT TTATTAGCCC TATCACCTAC TCTGCCTCCT ATAGCGCCTC TGGTGGCTCG TACCTCGCTG TGTACGGCTG GGTCAACTCT CCTCAGGCTG AATACCACGT TGTCGAGGCC TATGGTAATT ACAACCCTTG CAGCTCGGGC TCGGCTACAA ACCTTGGTAC CGTGTCCTCT GATGGAGGCA CCTACCAAGT CTGCACCGAC ACTCGGGTTA ACCAGCCATC GATAACGGGA ACGAGCACGT TCACGCAATT CTTCTCCGTT CGACAGGGCT CGCGCACATC TGGAACGGTG ACTATTGCCA ACCATTTCAA CTTCTGGGCG AAGCACGGCT TCGGCAACAG CAACTTCAAT TATCAGGTCG TAGCGGTAGA GGCATGGAGT GGTACTGGCA CCGCTAGTGT CACGGTCTCA GCTTAGGAGA TTAGGAGATG AGGGGCTTAG TGGTCAAAAA ACTTGAAAGC CGAGCTCTCT TGCAAGTGGT GTGATGATCG ACTAGGGGTT TAGCAATTTC AAACGTCGAG ACCGTATATA GTGTGGCGGG GCGGACGTGT CGCCGGTCGC GTTTTCAACT CACACAGACA ATTGACTCTC GTTTTCTCCG ATTTTGAAGT GTTTTATAAT AGTATTCACC CTATATTTGA TTAAATATGG CCTTATTCTC TAGGCCAGCC GACTTTTACC CCTATGCTAA TTTACATTTC TACCAACTAC CTCTTTTTGA AGCTATTTAC AACACCCCTC CGCTCCTATC TACACAATAC AGTGGGCTAC TGTGACTACA AGTAGCTGCG CCTACTCGCA TAGTTAGGAA AACAATCACA CTTGTACCTC AATTCAGGGT CTCAACTTCG CGAACGATAT GCAGGATACA ACTACTTTCT AAATACATAT TGATTGATCC CCGAGATTGT TCCAAACTGC CTGAACGGTG AACTTTGGGC CTCACCTGGT AAAGCCTGTC GTGGCCTTGT TGCCATTTCC GTTATTAGGC TGCCCCCATG GCGCGGCTAT AATGAGTTCC CATATAAGGC GGCCAACAGT TGTCCGCGGC TGGCTGCATC TCCACTAGGA ATCTCGAGAA TGCCGGTAAT GCTGAATCTA GGAACCTGGA CAACGACTGG GCGGCGGCTA CGGTCTTTGG GCGTTTGTGT TTTCGTTTCT CAATTTCAAA TGGCGACGGT TTGTTGTGGA AACGGCGACG AAGTTCTTCG ATGGTAAGTG TGGGGTTAGG AGGTGTGGGT CGCGGTGGCG GTGAAAATCT AGTAGGCTGC TGGAGAACAG AGGCGTTGGT ACGTTGTTGG CATTTGGGGG AGGTGATTGT CGGGGTTGGC TGTAGTTTAA GGTCGTGGAA AATAACTTGC TGAAAGCAGT TTTCTTTGTG TATCTTCGAT AAATCGCTGA TGAGGTTTCC CTGGATGGCT TTCAGCGGGA CCATAATCTC ACAGGTAAAG CGACAAGCTA TGTCGCCCAC TCCAGCGCGG ACTTGGTGAT AGAAGCCATT TTGGGCAAGT TGATGAGGGG TGATATCACT GAGGAGCTGG CGTTGAAATG AGATAGCTCT GGCTGCGATG GTCGAGTAGG TGGTTGAGAT ACGGAAGAGG GGCTTCACGT TGCGTCGTGG GGTGGTTCGG TTACACGGCA GGGTGGATTC GTTGGTGGGG TGGGAGGCTG CAGACGGGCT GGGAATGATT TTGCAGGGTC TCTGGTTCCC ACCAGGGCCT GGTTGATGGG AACTCATCCT CAGCCTGTCC TCAGCGTCTC CGATGTCCTG CGTACCGATG CATACAGGGA GGACTAAGGT AAGTTTCACC GCTTCTTTAA AGTGATTCTA TCTCAAATCT ACTGGGTGCC CACACTTTGC CCCTCCCTCG ACATTTGGGC GGTTAGTGAA AGGCAATACG GTAAATAGTG GACACTTCGC GCTGGCCACC AATCTGCCAA CTTGCACCTA CCAGGATATC ACGTCCTCCT AGCTTAAGAT GCGCCCACTG TTAGTGTCCA CAGAGGTCTC TCGACAGCAA GCATTCCCAA ATCAGAGACC ACTGCGCTCG TTGTGTCTCG GCTGTGACCC ACTTGAGCAG GCAATGAAGA CGATGGGTCA ATAGGGTCTT TCCTCATTAG AAGCGCGAGT GATCCACCGA ATCTTGCTCC GCAGCGGATT GTGCCTTCCA AGCAACACAT TGGCTCTCTA CCTGTCCAAA GCCGATTTTG GCCTGGGACA TATACAGAGA GGCTTCCTAT GACTTCAACT CTCTTGTATG GCATTTGCTC TCCGATCGGG CATCACGGGG CAAATTCCCT CTCAAAATGA TTCTCTGAGG AGGATTCGAC TCTCGGAAGA AAGGTTTTAT TCCGAGG
Cross-references
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GenBank AF359553
UniProt Q96W72
Protein AAK50762
RefSeq Protein
Broad
PDB
Entry history
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Entry Name XYN11B_PENPU
Previous Entry Names
Last Modification Date 2014-11-18